Product Introduction:

Liquid nitrogen, that is, liquid   Gaseous nitrogen is colorless and odorless.   A non-toxic gas. As an environmentally friendly refrigerant and industrial gas, liquid nitrogen is seeing increasingly widespread application across numerous fields, with broad uses in agriculture, healthcare, energy, materials science, and more.

Physical properties:

Liquid nitrogen Including it Inertia Colorless, odorless, Low viscosity 、 Non-corrosive 、 Non‑combustible , and Temperature Extremely low Properties: Liquid nitrogen has a boiling point of -196.56°C at standard atmospheric pressure and absorbs a large amount of heat when it vaporizes, potentially causing frostbite upon contact. In addition, liquid… Nitrogen's Density Its density is 0.81 g/cm³, and it appears as a colorless, transparent liquid. Liquid nitrogen also exhibits strong expansibility: 1 cubic meter of liquid nitrogen can expand to 696 cubic meters of pure gaseous nitrogen at 21°C. In industry, liquid nitrogen is produced through air separation—by liquefying air under pressure and cooling, then separating its components based on their differing boiling points.

Chemical properties:   

Chemical Properties of Liquid Nitrogen which is manifested in its Inactivity It neither burns nor supports combustion. This chemically inert nature means that liquid nitrogen exhibits unreactive behavior when undergoing chemical changes—specifically, it does not undergo chemical reactions with other substances. The chemical properties of liquid nitrogen also include its non‑toxicity and non‑flammability, characteristics that make it highly valuable in numerous applications, such as rapid freezing and transportation of food, cryoanesthesia, Material protector 、 Synthetic ammonia …such as the production of nitric acid.

Application areas:

01 Cryomedicine

Liquid nitrogen plays a crucial role in cryomedicine, particularly in the cryopreservation of biological samples and in transplantation applications. The low-temperature properties of liquid nitrogen make it an effective cooling agent, especially for reactions and the preservation of biological samples that require extremely low temperatures. During the phase transition when biological samples change from a liquid to a solid state, heat is released, causing the temperature to rise—potentially damaging cells. Therefore, accurately determining the phase transition point of biological samples and using microcomputers to program cooling protocols is of paramount importance. By increasing the flow rate of liquid nitrogen during the phase transition, temperature spikes can be mitigated, ensuring that cells safely navigate this critical period and thereby improving the viability of frozen samples.

02 Preserving Living Tissue

Liquid nitrogen plays a crucial role in the preservation of living tissues. Its ultra-low temperature properties make it an effective preservation method, capable of maintaining the viability and structural integrity of living tissues over extended periods. In addition, liquid nitrogen is used to store biological samples, as well as sperm and eggs, ensuring that they retain their original biological activity for long durations. When handling and storing liquid nitrogen, specific safety measures must be followed, such as providing adequate natural ventilation, carefully loading and unloading to prevent damage to cylinders and accessories, and ensuring that the storage area is equipped with leak emergency response equipment.

03 Welding and Metalworking

Liquid nitrogen plays a crucial role in welding and metalworking. Its cryogenic cooling capabilities make metals easier to shape during processing, such as when freezing cast pipe bends and extrusions. In addition, liquid nitrogen can be used for grinding, enhancing the surface finish of metals. These applications all benefit from liquid nitrogen’s low-temperature properties, which can effectively alter the physical state of metals and thereby optimize the machining process.

04 Transportation and Storage

Liquid nitrogen has a wide range of applications in transportation and storage. It is primarily used to store and transport biological samples, cells, tissues, and sperm, ensuring that these biological specimens maintain their viability and integrity over extended periods. In addition, liquid nitrogen can be used to store semiconductor materials and other chemical substances, providing them with a cryogenic environment to safeguard their quality and stability.

05 Maintain Food Quality

Liquid nitrogen is primarily used in the food industry to preserve food quality. Thanks to its extremely short freezing time, foods treated with liquid nitrogen can retain their original color, aroma, flavor, and nutritional value to the greatest extent possible. For example, Duan Zhenhua and his colleagues used liquid nitrogen to rapidly freeze betel nuts, and they found that the treated nuts not only had high chlorophyll content but also maintained their flavor exceptionally well. This rapid freezing technology is particularly important for foods that need to be transported or stored for long periods, as it ensures that the food remains fresh and delicious when it reaches consumers.

06 Storing Bioactive Samples

In the field of biomedicine, liquid nitrogen is primarily used for the storage of bioactive samples. Biological specimens such as vaccines, microbial and viral strains, cells, and animal organs can all be immersed in liquid nitrogen stored in liquid nitrogen tanks, enabling long‑term preservation of their viability. When needed, simply remove the sample, thaw it, and allow it to warm up—its original biological activity will then be restored. This method ensures that biological samples maintain their inherent biological activity and function over extended periods, providing significant convenience for biomedical research and treatment.

07 Frozen Storage

Liquid nitrogen plays a significant role in cryopreservation. It is widely used as a deep‑freezing agent, meaning it can rapidly and efficiently reduce temperatures, thereby preserving the original structure and properties of food, biological samples, and more. For example, liquid nitrogen can be employed to cryopreserve cells, tissues, or organs, ensuring that they remain viable over extended periods. In addition, liquid nitrogen can be used to quickly freeze food, extending its shelf life while maintaining its taste and nutritional value. Overall, liquid nitrogen’s cryopreservation capabilities make it an indispensable tool in both scientific research and industrial production.

08 Beauty Industry

Liquid nitrogen is widely used in the field of cosmetic medicine. As an extremely cold liquid, it can rapidly cool and solidify tissues, making it a common choice in cosmetic procedures to reduce bleeding, alleviate pain, and accelerate healing. In addition, liquid nitrogen can be employed in skin treatments such as cryotherapy to remove blemishes or lesions on the skin’s surface. This method is often used to treat warts, moles, or other skin conditions, allowing for precise removal of the affected area while minimizing damage to surrounding tissue. Overall, liquid nitrogen is a versatile and highly effective tool in the realm of cosmetic medicine.

Precautions for Use:

1. Safety Protection You must wear appropriate personal protective equipment, such as safety face shields, safety goggles, and insulated gloves, to prevent frostbite.

2. Placement of the Liquid Nitrogen Tank Liquid nitrogen tanks should be stored in a well‑ventilated, cool location, away from direct sunlight. Due to their precise manufacturing and inherent characteristics, liquid nitrogen tanks must never be tilted, laid horizontally, inverted, stacked, subjected to impact, or allowed to collide with other objects—whether in use or during storage. They should be handled with care and always kept upright.

3. Avoid liquid nitrogen splashing. Liquid nitrogen has a low boiling point; when it comes into contact with objects whose temperature is higher than its own, it will boil vigorously, vaporize, and even splash or spray. Therefore, care must be taken to prevent injuries caused by liquid nitrogen splashes.

4. Maintain ventilation In rooms where liquid nitrogen is used and stored, adequate ventilation must be maintained to prevent oxygen deficiency and asphyxiation.

5. Replenish liquid nitrogen in a timely manner. To ensure the viability of sperm or maintain other items at low temperatures, liquid nitrogen should be added to the cryogenic tank promptly, ensuring that the contents inside the tank are never exposed to the air outside.

6. Inspect the liquid nitrogen tank Regularly inspect the thermal insulation performance of the liquid nitrogen tank. If you notice frost forming on the outer surface of the tank or a decline in its insulation efficiency during use, stop using it immediately and replace it without delay. At the same time, ensure that the seals and lead seals on the vacuum valve, safety valve, and other components of the liquid nitrogen tank remain intact and undamaged.

7. Clean the liquid nitrogen tank In general, liquid nitrogen tanks should be cleaned and disinfected once a year. When cleaning, first scrub with a neutral detergent, then rinse thoroughly with warm water no hotter than 40°C. After draining all the water, use a blower to dry the tank completely; only after the inner liner is fully dry can liquid nitrogen be refilled.